Journal: ACS Sustainable Chemistry & Engineering

Article Title: Green Compressed Fluid Technologies To Extract Antioxidants and Lipids from Galdieria phlegrea in a Biorefinery Approach

doi: 10.1021/acssuschemeng.9b07505

Figure Lengend Snippet: Representative HPLC–DAD chromatograms of carotenoids extracted from G. phlegrea . (A) Conventional extraction of raw biomass; (B) conventional extraction of the residual biomass after PC extraction; (C) PLE extraction of the residual biomass after PC extraction. * indicates carotenoids and ** indicates chlorophylls. Peak numbers and their identification are reported in Table .

Article Snippet: All high pressure extractions were performed in a homemade compressed fluid extractor coupled to a PU-2080 HPLC pump from Jasco (Tokyo, Japan).

Techniques: Extraction

Journal: Molecules

Article Title: Contribution of TGF-β1 and Effects of Gene Silencer Pyrrole-Imidazole Polyamides Targeting TGF-β1 in Diabetic Nephropathy

doi: 10.3390/molecules25040950

Figure Lengend Snippet: Structure of pyrrole-imidazole (PI) polyamide targeting rat TGF-β1 and mismatch polyamide (Mismatch). PI polyamide targeting TGF-β1 was designed to span the boundary of the activator protein-1 (AP-1) binding site (Box: -2303 to -2297) of the TGF-β1 promoter. Mismatch was designed not to bind transcription binding sites of the promoter. Polyamides were synthesized by solid-phase methods and were purified by HPLC (0.1% AcOH/CH3CN 0 to 50% linear gradient, 0 to 40 min, 254 nm through a Chemcobond 5-ODS-H column).

Article Snippet: Finally, we purified the polyamides by high-performance liquid chromatography (HPLC) with use of a PU-980 HPLC pump, UV-975 HPLC UV/VIS detector (Jasco, Easton, MD, USA), and a Chemcobound 5-ODS-H column (Chemco Scientific, Osaka, Japan).

Techniques: Binding Assay, Synthesized, Purification